佐藤 利次 sato toshitsugu

学位

担当授業科目（学部）

基礎生物学　地球環境/短期履修, 基礎生物学　地域未来/短期履修, バイオ食品工学概論　地域未来（2020年度以前入学）, バイオ食品総合工学I　ﾊﾞｲｵ食品, 食品工学　ﾊﾞｲｵ食品, 食品衛生学　ﾊﾞｲｵ食品, バイオ食品工学演習　ﾊﾞｲｵ食品, 学術文献英語　ﾊﾞｲｵ食品, プレゼンテーション演習　ﾊﾞｲｵ食品, バイオ食品工学演習II　ﾊﾞｲｵ食品（2020年度以前入学）, バイオ食品総合工学II　ﾊﾞｲｵ食品, バイオ食品工学実験II　ﾊﾞｲｵ食品, 地域未来デザイン工学入門/短期履修, 地域未来デザイン工学入門　地域未来（2020年度以前入学）, コース概論　地域未来

担当授業科目（大学院）

生命科学特論IV　応用化学

専門分野

応用微生物学、食品科学、分子生物学

研究テーマ

シイタケによる有用酵素の高発現系の確立と分子育種, シイタケのラッカーゼの発現機構の解析, キノコ発酵農産物の解析, シイタケ栽培廃液に含まれる酵素の解析と環境浄化への応用, シイタケのゲノム編集技術の確立と子実体保存関連遺伝子の解析

研究内容キーワード

食用担子菌, 分子育種, シイタケ, 遺伝子導入, ラッカーゼ, 漆ラッカーゼ, キノコ発酵, 農産物, 抗酸化活性, ポリフェノール, シイタケ栽培廃液, 分子生物学・遺伝子工学 ・生物情報, 微生物学・ 応用微生物学, 機能性食品・食品科学, ゲノム編集

所属学会

日本農芸化学会, 日本生物工学会, 日本分子生物学会, 日本きのこ学会, 糸状菌分子生物学研究会, 北見医工連携研究会, 日本菌学会

学術論文

論文名／掲載誌名等			掲載年月
Stable transformation of Pleurotous ostreatus to hygromycin B resistance using Lentinus edodes GPD expression signals. Appl Microbiol Biotech, 56, 707-709			2001-09
Purification and characterization of an extracellular laccase from the edible mushroom Lentinula edodes, and decolorization of chemically different dyes. Appl Microbiol Biotech, 60, 327-335			2002-11
dffA gene from Aspergillus oryzae encodes L-ornithine N5-oxygenase and is indespensable for deferriferrichrysin biosynthesis. J Biosci Bioeng, 95, 1, 82-88			2003-01
Important role of fungal intracellular laccase for melanin synthesis: purification and characterization of an intracellular laccase from Lentinula　edodes fruit body. Microbiology, 149, 2455-2462			2003-09
Constructionn of a homologous selectable marker gene for Lentinula edodes transformation. Biosci Biotechnol Biochem, 67, 9, 2006-2009			2003-09
Isolation of genes specifically expressed in the fruit body of edible basidiomycete Lentinula edodes. Biosci Biotechnol Biochem, 68, 2, 468-472			2004-02
Bioremediation by Laccases from Lentinula edodes Mushroom Science, XVI, 573-578			2004-03
Isolation and characterization of a fruiting body specific exo-β-1, 3-glucanase-encoding gene, exg1, from Lentinula edodes Curr Genet, 47, 244-252			2005-02
Characterization of the Lentinula edodes exg2 gene encoding a lentinan-degrading exo-β-1, 3-glucanase. Curr Genet, 48, 195-203			2005-08
Lentinula edodes tlg1 encodes a thaumatin-like protein that is involved in lentinan degradation and fruiting body senescence. Plant Physiol, 141, 793-801			2006-06
Isolation and characterization of the gene encoding a manganese peroxidase from Lentinula edodes. Mycoscience, 48, 125-130			2007-04
Heterologous expression of lcc1 from Lentinula edodes in tobacco BY-2 cells results in the production an active, secreted form of fungal laccase Appl Microbiol Biotech, 79, 6, 971-980			2008-07
Isolation of industrial strains of Aspergillus oryzae lacking ferrichrysin by disruption of the dffA gene. J Biosci Bioeng, 106, 5, 488-492			2008-11
Cloning of Lentinula edodes Lemnp2 that encodes a manganese peroxidase secreted abundantly in sawdust medium Mycoscience, 50（2）, 116, 122			2009-03
シイタケ上面栽培廃液（上面水）のラッカーゼ製剤としての利用 日本きのこ学会誌, 17（1）, 11, 17			2009-04
The basidiomycetous mushroom Lentinula edodes white collar-2 homologue PHRB, a partner of putative blue-light photoreceptor PHRA, binds to a specific site in the promoter region of the L. edodes tyrosinase gene. Fungal Genet. Biol., 46 (4), 333, 341			2009-04
The tyrosinase-encoding gene of Lentinula edodes, Letyr, is abundantly expressed in the gills of the fruit-body during post-harvest preservation. Biosci Biotechnol Biochem, 73（5）, 1042, 1047			2009-05
Characterization of the post-harvest changes in gene transcription in the gill of the Lentinula edodes fruiting body. Curr. Genet., 55, 409, 423			2009-06
Purification of a novel extracellular laccase from the solid-state culture of the edible mushroom Lentinula edodes. Mycoscience, 50（4）, 308, 312			2009-07
シイタケの形質転換と子実体収穫後に発現する遺伝子 バイオサイエンスとバイオインダストリ, 67 (7), 316, 319			2009-09
Secretory expression of the non-secretory-type Lentinula edodes laccase by Aspergillus oryzae. Microbiol Res, 164, 642, 649			2009-11
A chimeric laccase with hybrid properties of the parental Lentinula edodes laccases. Microbiol Res, 165, 392-401			2010-07
Characterization of an extracellular laccase, PbLac1, purified from Polyporus brumalis. Fungal Biol., 114, 609-618			2010-08
Cloning and characterization of Lentinula edodes class II chitin synthase gene, LeChs2. Biosci. Biotechnol. Biochem., 78, 1707-1709			2010-08
Construction of novel vectors for transformation of Lentinula edodes using a chitin synthase gene promoter. J Biosci Bioeng, 111, 117-120			2011-02
Gene silencing of the Lentinula edodes lcc1 gene by expression of a homologous inverted repeat sequence. Microbiol Res			2011-09
Effective induction of pblac1 laccase by copper in Polyporus brumalis ibrc 5015. Fung Biol, 117, 52-61			2013-01
シイタケ菌床栽培初期における植物細胞壁分解酵素活性の変動と菌床中ラッカーゼの役割 日本きのこ学会誌, 22, 3, 114-120			2014-10
Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula erodes. Bioscience, Biotechnology, and Biochemistry, 81, 8, 1553-1556			2017-08
Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector Mycoscience, 60, 246-249			2019-07
Overexpression and repression of the tyrosinase gene in Lentinula edodes using the pChG vector J Biosci Bioeng, 128, 1, 1-7			2019-07
シイタケの遺伝子導入研究 きのこ研だより, 44, 10-22			2021-12
Disruption of the pyrG gene via CRISPR/Cas9-mediated homologous recombination and its use as a novel selectable marker gene in Lentinula edodes. FEMS Microbiology Letters, 370, 1-6			2023-06
Fermentation of soybeans with Pleurotus cornucopiae and Pleurotus ostreatus increases isoflavone aglycones, total polyphenol content,and antioxidant activity. Mycoscience, 64, 156-165			2023-10
Nutritional Characteristics of Edible Canna Leaves and 80% Methanol Extract before and after Lactic Acid Fermentation 日本食品保蔵科学会誌			2024-03

参考論文

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